| 货号 | 61101 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot/Immunofluorescence/Dot Blot/Immunocytochemistry |
| 使用方法 | Validated Applications: ChIP: 10 µg per ChIP ChIP-Seq: 4 µg each ICC/IF: 1 µg/ml dilution WB: 0.1 - 1 µg/ml dilution Published Applications: see references |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This Histone H3 trimethyl Lys36 antibody was raised against a peptide containing trimethyl Lys36 of human histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3 trimethyl Lys36 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from a human kidney. ChIP DNA was sequenced on the Illumina GA II and 30 million sequence tags were mapped to identify H3K36me3 binding across the genome. The image is zoomed in to show H3K36me3 binding across 5 different genes on chromosome 20. | |
Chromatin IP: ChIP performed using HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) and 10 µg of Histone H3 trimethyl Lys36 antibody (pAb) or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for either the PABPC1 (+ 0.5) or the PABPC1 ( +18) gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method. | |
Histone H3K36me3 antibody (pAb) tested by immunofluorescence. HeLa cells stained with Histone H3K36me3 antibody (green) at a 1 ug/ml dilution. Tubulin staining was done with our alpha Tubulin antibody, catalog no.39527 (red) at a 1:1,000 dilution. | |
Western Blot: Nuclear extract of HeLa cells (20 µg) probed with the Histone H3 trimethyl Lys36 antibody at a dilution of 1 µg/ml. | |
Specificity Data: Dot blot analysis was used to confirm the specificity of Histone H3 trimethyl Lys36 antibody for trimethyl-lysine 36 of histone H3. Peptides corresponding to regions around major sites of histone H3 methylation were spotted onto PVDF and probed with the antibody at a dilution of 1 µg/ml. The amount of peptide (in picomoles) spotted is indicated next to each row. Top panel - Lane 1: unmodified Lys4. Lane 2: monomethyl Lys4. Lane 3: dimethyl Lys4. Lane 4: trimethyl Lys4. Lane 5: unmodified Lys9. Lane 6: monomethyl Lys9. Lane 7: dimethyl Lys9. Lane 8: trimethyl Lys9. Lane 9: unmodified Lys79. Lane 10: monomethyl Lys79. Lane 11: dimethyl Lys79. Lane 12: trimethyl Lys79. Bottom panel - Lane 1: Unmodified Lys23. Lane 2: Monomethyl Lys23. Lane 3: Dimethyl Lys23. Lane 4: Trimethyl Lys23. Lane 5: unmodified Lys27. Lane 6: monomethyl Lys27. Lane 7: dimethyl Lys27. Lane 8: trimethyl Lys27. Lane 9: unmodified Lys36. Lane 10: monomethyl Lys36. Lane 11: dimethyl Lys36. Lane 12: trimethyl Lys36. |
