| 货号 | 39917 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot/Immunofluorescence/Dot Blot/Immunocytochemistry |
| 使用方法 | Validated Applications: ChIP: 10 µg per ChIP ChIP-Seq: 3 µg each ICC/IF: 2 µg/ml dilution WB: 0.5 - 2 µg/ml dilution NGS-QC® certification. This antibody has been processed by the NGS-QC® generator. For additional details, click here.. Published Applications: ChIP See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif | |
| 纯化方式 | Protein A Chromatography | |
| 免疫原 | This Histone H3 acetyl Lys9 antibody was raised against a peptide including acetyl-lysine 9 of histone H3. | |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. | |
| 运输条件 | Blue Ice | |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. | |
| 存储溶液 | Purified IgG in PBS (pH 7.5) with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3 Acetyl Lys9 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from mouse liver. ChIP DNA was sequenced on the Illumina GA II and 25 million sequence tags were mapped to identify H3K9Ac binding across the genome. The image shows a 1.5 million base pair region on chromosome 15. H3K9Ac shows promoter localization at many genes and broader binding near the Gcat gene. | |
ChIP of Histone H3 acetyl Lys9 pAb. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 3 µg of Histone H3 acetyl Lys9 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method. | |
Immunofluorescence stain of Histone H3 acetyl Lys9 pAb. HeLa cells stained at 2 µg/ml with Histone H3 acetyl Lys9 antibody. Top panel: Histone H3 acetyl Lys9 antibody. Middle panel: DAPI. Bottom panel: merge. | |
Western blot of Histone H3 acetyl Lys9 antibody (pAb). HeLa nuclear extract (20 µg per lane) probed with Histone H3 acetyl Lys9 antibody (1 µg per ml). Lane 1: no treatment. Lane 2: cells treated with sodium butyrate. | |
Histone H3 acetyl Lys9 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys9 antibody for acetyl Lys9 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 acetyl Lys9 antibody at a dilution of 1 µg/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: histone H3 acetyl-Lys4 peptide. Lane 2: unmodified Lys4 peptide. Lane 3: acetyl-Lys18 peptide. Lane 4: unmodified Lys9/14/18 peptide. Lane 5: acetyl-Lys9 peptide. Lane 6: acetyl- Lys14 peptide. Lane 7: acetyl-Lys18 peptide. Lane 8: acetyl-Lys23 peptide. Lane 9: acetyl-Lys27 peptide. Lane 10: unmodified Lys27 peptide. |
