| 货号 | 39715 |
| 同种亚型 | IgG |
| 反应种属 | Drosophila |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot |
| 使用方法 | Validated Applications: ChIP: 4 - 6 µl per ChIP ChIP-Seq & ChIP-chip: 4 - 6 µl each WB: 1:500 - 1:1,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This Histone H2Av antibody was raised against a peptide in the C-terminus of the Drosophila melanogaster histone variant H2Av. |
| 背景 | The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H2A.Z(H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle.In Drosophila, the H2A variant corresponding to H2AZ is H2Av.H2Av is an essential protein in Drosophila and has been implicated in both activation and repression of transcription. H2Av is localized to centromeric heterochromatin in Drosophila and flies lacking H2Av have reduced levels of heterochromatin components at the centromeres. However, H2Av nucleosome distribution throughout the rest of the Drosophila genome correlates with genes that have an open and uniform chromatin architecture at promoter regions. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 15 kDa |
| 参考文献 |
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Histone H2Av antibody tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 10 million Drosophila cells and 4 µg of H2Av antibody. ChIP DNA was sequenced on the Illumina HiSeq and 8 million sequence tags were mapped to identify H2Av binding patterns. The image on the left shows H2Av binding within a 0.5 million bp region on Drosophila chromosome 3L. The middle and right images show binding at 3 representative genes. A common noted feature is signal depletion within introns as illustrated at Gene CG7047 and CG2678. Another noted characteristic is that the highest signal tends to occur at promoters with diminishing signal related to proximity to the 3´ end of genes. | |
Histone H2Av antibody tested by Western blot. Detection of Histone H2Av by Western blot analysis. Whole cell extract from Drosophila third instar larvae blotted with Histone H2Av antibody at a dilution of 1:500. |
