| 货号 | 39281 |
| 同种亚型 | IgG |
| 反应种属 | Budding Yeast, Human |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot/Dot Blot |
| 使用方法 | Validated Applications: ChIP: 3 - 5 µl per ChIP ChIP-Seq: 10 µl each WB: 1:2,500 - 1:5,000 dilution Published Applications: ChIP WB See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This Histone H3 acetyl Lys56 antibody was raised against peptide containing acetyl-Lys56 of yeast histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H3 Lys56 acetylation occurs normally during S phase, but disappears in G2. This modification persists in presence of DNA damage and also plays a role in nucleosome assembly. Rtt109 was shown to be the major histone acetyltransferase (HAT) for Lys56 acetylation. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified rabbit IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3K56ac antibody (pAb) tested by ChIP-chip. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 3 million HeLa cells and 5 µl of antibody. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image on the left shows H3K56Ac binding across a 650,000 bp region on chromosome 17. The image on the right shows H3K56Ac binding at the KLF5 gene. | |
Histone H3 acetyl Lys56 pAb tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 2 µl of Histone H3 acetyl Lys56 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method. | |
Histone H3 acetyl Lys56 antibody tested by Western blot. HeLa acid extract (10 µg/lane) was probed with Histone H3 acetyl Lys56 polyclonal antibody (1:1,000 dilution). Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate. | |
Histone H3 acetyl Lys56 antibody tested by Western blot. Acid extract of yeast cells probed with Histone H3 acetyl Lys56 pAb (1:1,400 dilution). |
