| 货号 | 39155 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot/Immunofluorescence/Dot Blot/Immunocytochemistry |
| 使用方法 | Validated Applications: ChIP: 5 - 10 µg per ChIP ChIP-Seq: 5 µg each ICC/IF: 2 µg/ml dilution WB: 0.5 - 2 µg/ml dilution Published Applications: ChIP-Seq See references for more information. Individual optimization may be required. The modENCODE and NIH Roadmap Epigenomics Mapping Consortiums have implemented rigorous standardization criteria for all assays and reagents to be used. As part of this initiative, antibody specificity testing and the ability of the antibodies to work in ChIP-Seq were assessed in a large-scale study. This Histone H3 trimethyl Lys27 antibody was validated for ChIP-Seq in the study (see reference). |
| 供应商 | Active Motif | |
| 纯化方式 | Protein A Chromatography | |
| 免疫原 | This Histone H3 trimethyl Lys27 antibody was raised against a peptide including trimethyl-lysine 27 of histone H3. testing | |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or H3K27me3) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression. | |
| 运输条件 | Blue Ice | |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. | |
| 存储溶液 | Purified IgG in PBS (pH 7.5) with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3 trimethyl Lys27 pAb tested by ChIP-Seq. ChIP was performed using chromatin from the human iPS 19.11 cell line. ChIP DNA was sequenced on the Illumina GA II and sequence tags were mapped to identify H3K27me3 binding. The image shows H3K27me3 binding across a 140,000 bp region of the HOX gene cluster on chromosome 12. | |
Histone H3 trimethyl Lys27 antibody tested by immunofluorescence. HeLa cells stained with Histone H3 trimethyl Lys27 antibody (1.7 µg/ml dilution). Top panel: Histone H3 trimethyl Lys27 antibody. Middle: DAPI. Bottom: Merge of both images. | |
Histone H3 trimethyl Lys27 antibody tested by Western blot. HeLa acid extract (20 µg) probed with Histone H3 trimethyl Lys27 antibody (1 µg/ml). | |
Histone H3 trimethyl Lys27 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 trimethyl Lys27 antibody for trimethyl-lysine 27 of histone H3. Peptides corresponding to regions around major sites of histone H3 methylation (lysine 4, lysine 9, lysine 27) were spotted onto PVDF and probed with the antibody at a dilution of 1 µg/ml. The amount of peptide (in picomoles) spotted is indicated next to each row. Lane 1: Unmodified Lys4. Lane 2: Monomethyl Lys4. Lane 3: Dimethyl Lys4. Lane 4: Trimethyl Lys4. Lane 5: Monomethyl Lys9. Lane 6: Unmodified Lys9. Lane 7: Dimethyl Lys9. Lane 8: Trimethyl Lys9. Lane 9: Unmodified Lys27. Lane 10: Monomethyl Lys27. Lane 11: Dimethyl Lys27. Lane 12: Trimethyl Lys27. |
