Histone H3K27ac antibody (pAb)【科瑞奥博】

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Histone H3K27ac antibody (pAb)

货号： 39135 基本售价： 6307.5 元规格： 100 ul

产品信息

概述
货号	39135
同种亚型	Serum
反应种属	Budding Yeast, Human, Other (Wide Range)
来源宿主	Rabbit
应用	ChIP/ChIP-Seq/Western Blot/Dot Blot
使用方法	Validated Applications: ChIP: 3 µl per ChIP ChIP-Seq: 5 µl each WB: 1:1,000 - 1:5,000 dilution Published Applications: ChIP-Seq See references for more information. Individual optimization may be required. The modENCODE and NIH Roadmap Epigenomics Mapping Consortiums have implemented rigorous standardization criteria for all assays and reagents to be used. As part of this initiative, antibody specificity testing and the ability of the antibodies to work in ChIP-Seq were assessed in a large-scale study. Histone H3 acetyl Lys27 antibody was validated for ChIP-Seq in the study (see reference).

性能
供应商	Active Motif
纯化方式	None
免疫原	This Histone H3 acetyl Lys27 antibody was raised against a peptide including acetyl-lysine 27 of histone H3.
背景	Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone acetylation is often associated with transcriptional activation and acetylation of H3K27 is found at active enhancers. Lysine 27 of histone H3 can also be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.
运输条件	Blue Ice
存放说明	Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
存储溶液	Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an
计算分子量	17 kDa
参考文献

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参考图片

Histone H3 acetyl Lys27 antibody tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT^® Express Kit (Catalog No. 53008) and HeLa Chromatin (2.1 x 10⁶ cell equivalents per ChIP) using 3 µl of Histone H3 acetyl Lys27 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using primer pairs specific for the indicated gene as well as a negative control primer pair. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

Histone H3 acetyl Lys27 antibody tested by Western blot.
HeLa acid extract probed with Histone H3 acetyl Lys27 polyclonal antibody (1:1,000 dilution).
Lane 1: No treatment.
Lane 2: Cells treated with sodium butyrate.

Histone H3 acetyl Lys27 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys27 pAb for acetyl Lys27 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: Acetyl-Lys9 peptide. Lane 2: Unmodified Lys9 peptide. Lane 3: Acetyl-Lys14 peptide. Lane 4: Unmodified Lys14 peptide. Lane 5: Acetyl-Lys18 peptide. Lane 6: Unmodified Lys18 peptide. Lane 7: Acetyl-Lys23 peptide. Lane 8: Unmodified Lys23 peptide. Lane 9: Acetyl-Lys27 peptide. Lane 10: Unmodified Lys27 peptide.