| 货号 | 39255 |
| 同种亚型 | Serum |
| 反应种属 | Budding Yeast, Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot/Immunofluorescence/Dot Blot/Immunocytochemistry |
| 使用方法 | Validated Applications: ChIP: 3 - 5 µl per ChIP ChIP-Seq: 10 µl each ICC/IF: 1:250 - 1:1,000 dilution WB: 1:2500 - 1:5,000 dilution Published Applications: ICC/IF WB See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif | |
| 纯化方式 | None | |
| 免疫原 | This Histone H3 dimethyl Lys36 antibody was raised against a peptide including dimethyl-lysine 36 of histone H3. | |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes. | |
| 运输条件 | Blue Ice | |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. | |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an 计算分子量 | 17 kDa |
| 参考文献 |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Histone H3 dimethyl Lys36 antibody tested by ChIP-chip. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from a human lymphoma cell line (3 million cells). The ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image shows H3K36me2 enrichment across the FMNL2 gene. | |
Histone H3 dimethyl Lys36 antibody tested by immunofluorescence. Staining of HeLa cells with Histone H3 dimethyl Lys36 antibody (1:500 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel). | |
Histone H3 dimethyl Lys36 antibody tested by Western blot. HeLa acid extract (10 µg per lane) probed with Histone H3 dimethyl Lys36 antibody at a dilution of 1:2,500. | |
Histone H3 dimethyl Lys36 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Lys36 antibody for histone H3 dimethyl Lys36. Methylated peptides corresponding to the immunogen were spotted onto PVDF and probed with the antibody at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: trimethyl-Lys36 peptide. Lane 2: dimethyl-Lys36 peptide. Lane 3: unmodified Lys36 peptide. |
