| 货号 | 39245 |
| 同种亚型 | Serum |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/Western Blot/Immunofluorescence/Dot Blot/Immunocytochemistry |
| 使用方法 | Validated Applications: ChIP: 5 - 10 µl per ChIP ICC/IF: 1:200 - 1:500 dilution WB: 1:500 - 1:1,000 dilution Published Applications: see references |
| 供应商 | Active Motif | |
| 纯化方式 | None | |
| 免疫原 | This Histone H3 dimethyl Lys27 antibody was raised against a peptide including dimethyl-lysine 27 of histone H3. | |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or Histone H3 trimethyl Lys27) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression. | |
| 运输条件 | Blue Ice | |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. | |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3 dimethyl Lys27 antibody tested by ChIP. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and 50 µl of Ready-to-ChIP HeLa Chromatin (Catalog No. 53015) per ChIP. Subsequent to the ChIP reaction, DNA was purified from the immunoprecipitated chromatin and a region approximately 1000 base pairs 5′ of the transcriptional start site of the HoxD13 gene was amplified by PCR. Lane 1: ChIP using negative control rabbit IgG. Lane 2: ChIP using 10 µl of Histone H3 dimethyl Lys27 antibody. Lane 3: PCR input control. | |
Histone H3 dimethyl Lys27 antibody tested by immunofluorescence. Staining of HeLa cells with Histone H3 dimethyl Lys27 antibody (1:500 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel). | |
Histone H3 dimethyl Lys27 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Lys27 antibody for dimethyl Lys27 histone H3. Methylated peptides corresponding to the immunogen and related sequences derived from histone H3 were spotted onto PVDF and probed with the antibody at a 1:5,000 dilution. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Unmodified lysine 4 peptide. Lane 2: Monomethyl lysine 4 peptide. Lane 3: Dimethyl lysine 4 peptide. Lane 4: Trimethyl lysine 4 peptide. Lane 5: Unmodified lysine 9 peptide. Lane 6: Monomethyl lysine 9 peptide. Lane 7: Dimethyl lysine 9 peptide. Lane 8: Trimethyl lysine 9 peptide. Lane 9: Unmodified lysine 27 peptide. Lane 10: Monomethyl lysine 27 peptide. Lane 11: Dimethyl lysine 27 peptide. Lane 12: Trimethyl lysine 27 peptide. |
