| 货号 | 39119 |
| 别名 | Histone H2B acetyl-lysine 120 |
| 同种亚型 | Serum |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot/Dot Blot |
| 使用方法 | Validated Applications: ChIP: 10 µl per ChIP ChIP-Seq: 5 µl each WB: 1:1,000 - 1:2,000 dilution Published Applications: ChIP-Seq See references for more information. Individual optimization may be required. The modENCODE and NIH Roadmap Epigenomics Mapping Consortiums have implemented rigorous standardization criteria for all assays and reagents to be used. As part of this initiative, antibody specificity testing and the ability of the antibodies to work in ChIP-Seq were assessed in a large-scale study. This Histone H2B acetyl Lys120 antibody was validated for ChIP-Seq in the study (see reference). |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This Histone H2B acetyl Lys120 antibody was raised against a peptide including acetyl-lysine 120 of human histone H2B. |
| 背景 | Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H2A and Histone H2B are acetylated in bulk chromatin by p300 and form acetylated Histone H2A/Histone H2B heterodimers. When DNA associates with intact core histone octamers that contain acetylated H2A/H2B dimers, the inhibition of transcriptional initiation significantly decreases, indicating that acetylation of their lysine residues may mediate transcription. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 15 kDa |
| 参考文献 |
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Histone H2B acetyl Lys120 pAb tested by ChIP-Seq. ChIP was performed using chromatin from the H1 human embryonic stem cell line. ChIP DNA was sequenced on the Illumina GA II and sequence tags were mapped to identify H2BK120Ac binding. The image shows H2BK120Ac binding in and around the IGSF21 gene on chromosome 1. | |
Histone H2B acetyl Lys120 pAb tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µl of Histone H2B acetyl Lys120 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method. | |
Histone H2B acetyl Lys120 pAb tested by Western blot. HeLa acid extract (5 µg per lane) was probed with Histone H2B acetyl Lys120 polyclonal antibody (1:2,000 dilution). Lane 1: Human recombinant H2B (200 ng). Lane 2: No treatment. Lane 3: Cells treated with sodium butyrate. | |
Histone H2B acetyl Lys120 pAb tested by dot blot analysis. Specificity Data: Dot blot analysis was used to confirm the specificity of Histone H2B acetyl Lys120 pAb for acetyl-Lys 120 of histone H2B. Decreasing amounts of modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:2,000. Lane 1: Peptide acetylated at lysine 5 of H2B. Lane 2: Unmodified lysine 5 peptide. Lane 3: Peptide acetylated at lysine 16 of H2B. Lane 4: Unmodified lysine 16 peptide. Lane 5: Peptide acetylated at lysine 120 of H2B. Lane 6: Unmodified lysine 120 peptide. |
