| 货号 | 39113 |
| 同种亚型 | Serum |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot/Immunofluorescence/Dot Blot |
| 使用方法 | Validated Applications: ChIP: 2 - 10 µl per ChIP ChIP-Seq: 5 µl each WB: 1:1,000 - 1:5,000 dilution Published Applications: ChIP ChIP-Seq WB IF See references for more information. Individual optimization may be required. This histone variant can be perturbed by excessive washing steps prior to fixation. Cells should be washed into serum-free culture medium (NOT PBS!) prior to fixation. |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This Histone H2A.Z antibody was raised against a peptide derived from the C-terminus of human histone H2A.Z. |
| 背景 | Thenucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle. H2A.Z is acetylated at multiple lysine residues in its amino terminus, which may serve to allow H2A.Z to function as an insulator of chromatin domains. |
| 运输条件 | Blue Ice |
| 存放说明 | Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an Ig |
| 计算分子量 | 14 kDa |
| 参考文献 |
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