| 货号 | 61785 |
| 克隆类别 | 1G6-E3 |
| 同种亚型 | IgG1 |
| 反应种属 | Drosophila |
| 来源宿主 | Mouse |
| 应用 | ChIP/ChIP-Seq/Western Blot/Immunoprecipitation/Immunofluorescence |
| 使用方法 | Validated Applications: Western Blot: 0.2 - 1 µg/ml Published Applications: ChIP-Seq ChIP IP IF WB See references for more information. Individual optimization may be required. References Iwasakiet. al., 2016, Molecular Cell 63, p. 408-419 |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This antibody was raised against a recombinant protein containing amino acids 109-256 of Drosophila melanogaster Histone H1. This region of the protein is not conserved in mammals; the antibody is not likely to cross-react to human, mouse or rat Histone H1. |
| 背景 | The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation and play a major role in regulating gene expression. |
| 运输条件 | Blue Ice |
| 存放说明 | Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 35 kDa |
| 参考文献 |
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