| 货号 | 61617 |
| 同种亚型 | IgG |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Dot Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:1,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This antibody was raised against a peptide containing phospho-tyrosine 142 of human histone H2A.X. |
| 背景 | Histone H2A.XY142ph (H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation.Tyrosine phosphorylation at position 142 on Histone H2A.X is constitutive and is mediated by BAZ1B/WSTF. This mark distinguishes between apoptotic and DNA repair responses to genotoxic stress. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 15 kDa |
| 参考文献 |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Histone H2A.XY142ph antibody (pAb) tested by Western blot. 25 µg of U2OS nuclear extract untreated (lanes 1, 3, 5) or treated with 5 µM camptothecin for 8 hours (lanes 2, 4, 6) probed with the indicated antibody. Lanes 1 & 2: H2A.XY142ph antibody (pAb) at a 1:500 dilution (expected decrease with treatment). Lanes 3 & 4: H2A.X antibody (pAb), catalog no 39689 at a 1:50,000 dilution (no change expected). Lanes 5 & 6: H2A.XS139ph antibody (pAb), catalog no 39117 at a 1:20,000 dilution (increase expected with treatment). | |
Histone H2A.XY142ph antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H2A.XY142ph antibody for phospho Tyr142 of Histone H2A.X. Peptides corresponding to the immunogen and the unmodified version of the immunogen were spotted onto PVDF and probed with the antibody at 1:100,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Phospho-Tyr142 peptide. Lane 2: Unmodified Tyr142 peptide. Lane 3: Phospho-Ser139 peptide. Lane 4: Unmodified Ser139 peptide. |
