| 货号 | 61613 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/Western Blot/Dot Blot |
| 使用方法 | Validated Applications: ChIP: 10 µl per ChIP WB: 1:500 - 1:1,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This antibody was raised against a synthetic peptide containing asymmetric dimethyl arginine 17 and acetyl lysine 18 of human Histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residues. Acetylation of histones occurs on lysine and is typically associated with activation of gene expression. Co-occurrence of two modifications adds an additional layer of complexity and regulation to the function of these modifications. Dual modification of H3R17me2aK18ac may play a role in enhancer function. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 17 kDa |
| 参考文献 |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Histone H3R17me2aK18ac antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed with 5 x 106 cell equivalents of chromatin from a murine HAFTL pro-B cell line and 10 µl of Histone H3R17me2aK18ac antibody. ChIP DNA was used in qPCR with a negative control primer pair (Neg) or enhancer-specific primer pairs that are “off” in B cells (Cd14) or “on” in B cells (IL7r), as indicated. This data shows that the dual modification R17me2aK18ac on Histone H3 is enriched in active enhancers. The data are presented as Fold over Input. | |
Histone H3R17me2aK18ac antibody (pAb) tested by Western blot. Nuclear extract of Raji cells (20 µg per lane) was probed with H3R17me2aK18ac (pAb) at a 1:500 dilution. Lane 1: Untreated cells. Lane 2: Cells treated with sodium butyrate. | |
Histone H3R17me2aK18ac antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3R17me2aK18ac antibody for asymmetric dimethyl-arginine 17 and acetyl-lysine 18 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with H3R17me2aK18ac antibody at a 1:500 dilution. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Peptide containing asymmetric dimethyl-Arg17 and acetyl-Lys18 of Histone H3 . Lane 2: unmodified Histone H3 peptide. Lane 3: Asymmetric dimethyl-Arg17 of Histone H3 peptide. Lane 4: Acetyl-Lys18 of Histone H3 peptide. |
