| 货号 | 61605 |
| 同种亚型 | IgG |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Dot Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:1,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This antibody was raised against a peptide containing human histone H2AQ104me1. |
| 背景 | Histone H2A is one of the core components of the nucleosome, the basic building block of chromatin. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (Histone H2A, Histone H2B, Histone H3, and Histone H4). Methylation on glutamine 104 has been implicated in RNA Pol-I transcriptional regulation. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 14 kDa |
| 参考文献 |
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Histone H2AQ104me1 antibody (pAb) tested by Western blot. Histone H2AQ104me1 detection by Western blot. The analysis was performed using 30 µg of HeLa nuclear cell extract and Histone H2AQ104me1 antibody at a 1:500 dilution. | |
Histone H2AQ104me1 (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H2AQ104me1 pAb for monomethyl-glu104 of histone H2A. Decreasing amounts of modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. Lane 1: Unmodified glutamine 104 of H2A peptide. Lane 2: Monomethylated glutamine 104 of H2A peptide. |
