| 货号 | 61483 |
| 别名 | CenH3, CENPA |
| 同种亚型 | IgG |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunofluorescence/Dot Blot/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 1:100 dilution WB: 1:100 - 1:500 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This antibody was raised against a synthetic peptide containing phosphorylated serine 16 and 18 of human CENP-A. |
| 背景 | CENP-A (Centromere Protein A) is a Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. It is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. Recently, phosphorylation of CENP-A on Ser16 and Ser18 was reported and found to be important for chromosome segregation during mitosis. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 16 kDa |
| 参考文献 |
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CENP-A phospho Ser18 antibody (pAb) tested by immunofluorescence. Detection of CENP-A phospho Ser18 by immunofluorescence. HeLa cells were stained with CENP-A phospho Ser18 antibody at a 1:100 dilution. Green: CENP-A phospho Ser18 antibody (pAb) Blue: DAPI | |
CENP-A phospho Ser18 antibody (pAb) tested by Western blot. Top: Recombinant CENP-A (20 ng) was probed with CENP-A phospho Ser18 antibody (1:100 dilution) (A) or with anti-centromere antibody (ACA) (B). CENP-A phospho Ser18 antibody does not detect recombinant CENP-A. Bottom: HeLa nuclear extract (20 µg) was probed with CENP-A phospho Ser18 antibody (1:250 dilution). Lane 1: CENP-A phospho-Ser18 antibody. Lane 2: anti-centromere antibody (ACA). | |
CENP-A phospho Ser18 antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of CENP-A phospho Ser18 antibody. Peptides corresponding to the immunogen and related proteins were spotted onto PVDF and probed with the antibody at 1:750. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: CENP-A phospho Ser 16 and phospho Ser18. Lane 2: CENP-A unmodified. Lane 3: CENP-A phospho Ser 16. Lane 4: CENP-A phospho Ser 18. |
