| 货号 | 61439 |
| 同种亚型 | IgG |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Dot Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:1,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This antibody was raised against a synthetic peptide containing monomethyl Lysine 5 of human histone H2B. |
| 背景 | Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Changes in methylation of histone H2B may be involved in heat-shock mechanisms. Monomethylation of Lysine 5 has been reported to occur at active promoters downstream of the TSS. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 15 kDa |
| 参考文献 |
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Histone H2BK5me1 (pAb) tested by Western blot. HeLa acid extract (20 µg) probed with Histone H2BK5me1 pAb (1:500 dilution). | |
Histone H2BK5me1 (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H2BK5me1 pAb for monomethyl-Lys5 of histone H2B. Decreasing amounts of modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:10,000. Lane 1: Unmodified lysine 5 peptide. Lane 2: Peptide monomethylated at lysine 5 of H2B. Lane 3: Peptide dimethylated at lysine 5 of H2B. Lane 4: Peptide trimethylated at lysine 5 of H2B. |
