| 货号 | 61423 |
| 别名 | PK3, TCB, OIP3, PKM, CTHBP, THBP1, HEL-S-30 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:2,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This antibody was raised against a peptide within the C-terminal region of human PKM2. |
| 背景 | PKM2 (pyruvate kinase M2 isoform) is a glycolytic enzyme that catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues. The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development, is upregulated in human cancer cells and has been implicated in transcriptional regulation. Recently PKM2 has been described to bind to Histone H3 and phosphorylate threonine 11 (H3T11ph) which implicates PKM2 as an epigenetic regulator of gene expression. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol, 0.035% sodium azide and 0.2 mg/ml BSA. |
| 计算分子量 | 60 kDa |
| 参考文献 |
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PKM2 pAb tested by Western blot. PKM2 detection by Western blot. The analysis was performed using 30 µg HeLa whole cell extract and PKM2 at a 1:1,000 dilution. |
