| 货号 | 39737 |
| 同种亚型 | IgG |
| 反应种属 | Budding Yeast, Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Dot Blot |
| 使用方法 | Validated Applications: WB: 1:2,000 - 1:10,000 dilution This modification is extremely labile so non-denatured samples should not be allowed to sit in aqueous buffer. For Western blotting, overnight transfer at low voltage is recommended. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This Histone H3 phospho Thr45 antibody was raised against a peptide containing phospho Thr45 of human histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of histone H2A, histone H2B, histone H3 and histone H4). Histones are subject to a number of post-translational modifications, including acetylation, phosphorylation and methylation. In budding yeast, H3 Thr45 phosphorylation occurs specifically during DNA replication, and is catalyzed by the Cdc7–Dbf4 kinase. Yeast that lack threonine 45 exhibit defects in replication, suggesting that phosphorylation of Thr45 is associated with DNA replication. Threonine 45 lies within a conserved region of histone H3 that makes critical contacts with DNA when assembled into chromatin. It is located at the points of entry and exit of DNA on the nucleosome, and phosphorylation may influence histone-DNA interactions within the nucleosome. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3 phospho Thr45 antibody (pAb) tested by Western blot. Yeast extracts from a wild type strain (Lane 1) and a strain in which threonine 45 has been mutated to an alanine (T45A, Lane 2) were blotted and probed with Histone H3 phospho Thr45 antibody (pAb) at a dilution of 1:10,000. The arrow represents the migration of histone H3. Note that both the upper and lower bands disappear in the T45A mutant, suggesting that the upper band represents a post-translational modification of histone H3 that causes a dramatic decrease in gel mobility. | |
Histone H3 phospho Thr45 antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 phospho Thr45 antibody (pAb) for Histone H3 phospho Thr45. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 phospho Thr45 antibody (pAb) at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row Lane 1: phospho Thr45 peptide. Lane 2: unmodified Thr45 peptide. Lane 3: phospho Thr3 peptide. Lane 4: phospho Thr6 peptide. Lane 5: phospho Thr11 peptide. Lane 6: phospho Thr80 peptide. Lane 7: phospho Ser10 peptide. Lane 8: phospho Ser28 peptide. |
