| 货号 | 39703 |
| 同种亚型 | IgG |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Dot Blot |
| 使用方法 | Validated Applications: WB: 1:500 dilution Published Applications: see references |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This Histone H3 dimethyl Arg2 (H3R2me2s) antibody was raised against a peptide containing dimethyl-Arg2 (symmetric) of human histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 50% glycerol and 0.02% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3 dimethyl Arg2 symmetric pAb tested by Western blot. Western blot performed using Histone H3 dimethyl Arg2 symmetric pAb at a dilution of 1:500. Lane 1: Nuclear extract from HEK293 cells (25 µg). Lane 2: Nuclear extract from HeLa cells (20 µg). | |
Histone H3 dimethyl Arg2 symmetric pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Arg2 symmetric pAb for dimethyl-arginine 2 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 dimethyl Arg2 symmetric pAb at 1:10,000. The amount of peptide (picomoles) spotted is indicated next to each row. Top Panel: Lane 1: unmodified Arg2. Lane 2: monomethyl-Arg2 H3. Lane 3: dimethyl-Arg2 H3 (symmetric). Lane 4: dimethyl-Arg2 H3 (asymmetric). Lane 5: unmodified Arg8. Lane 6: monomethyl-Arg8 H3. Lane 7: dimethyl-Arg8 H3 (symmetric). Lane 8: dimethyl-Arg8 H3 (asymmetric).Bottom Panel: Lane 1: unmodified Arg17 H3. Lane 2: monomethyl-Arg17. Lane 3: dimethyl-Arg17 H3 (symmetric). Lane 4: dimethyl-Arg17 H3 (asymmetric). Lane 5: unmodified Arg26. Lane 6: monomethyl-Arg26 H3. Lane 7: dimethyl-Arg26 H3 (symmetric). Lane 8: dimethyl-Arg26 H3 (asymmetric). |
