| 货号 | 39637 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Dot Blot |
| 使用方法 | Validated Applications: WB: 1:500 dilution Published Applications: see references |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This Histone H3.3 phospho Ser31 antibody was raised against a peptide containing phospho Ser31 of Histone H3.3. |
| 背景 | Histone H3.3(H3F3) is a variant of histone H3 that contains a serine (S) to alanine (A) replacement at amino acid position 31. This variant Histone H3.3 has been found to replace conventional histone H3 in nucleosomes of active genes. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Histone variants differ in amino acid sequence from their core histone counterparts and in some cases have also been shown to have functional differences to the more typical histones. Histone variant H3.3 is the predominant form of histone H3 in non-dividing cells. Phosphorylation of serine 31 of histone variant H3.3 is specific to regions bordering centromeres in metaphase chromosomes. (Reference: Hake et al. (2005) PNAS 102: 6344-6349.) |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3.3 phospho Ser31 antibody tested by Western blot. Detection of Histone H3.3 by Western blot. The analysis was performed using 40 µg of untreated (lane 1) or cocemid treated (lane 2) HeLa acid extract with a 1:500 dilution of Histone H3.3 phospho Ser31 antibody. | |
Histone H3.3 phospho Ser31 antibody tested by Dot blot. Dot blot analysis was used to confirm the specificity of Histone H3.3 phospho Ser31 antibody. Peptides corresponding to histone H3.3 and regions around major sites of histone H3 phosphorylation (serine 10 and serine 28) were spotted onto PVDF and probed with 39637 at a dilution of 1:10,000. The amount of peptide (in picomoles) spotted is indicated next to each row. Lane 1: peptide phosphorylated at serine 31 of H3.3. Lane 2: unmodified serine 31 peptide. Lane 3: peptide phosphorylated at serine 10 of H3. Lane 4: peptide phosphorylated at serine 28 of H3. |
