| 货号 | 39587 |
| 同种亚型 | Serum |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/Western Blot/Immunofluorescence/Dot Blot/Immunocytochemistry |
| 使用方法 | Validated Applications: ChIP: 5 - 10 µl per ChIP ICC/IF: 1:500 - 1:1,000 dilution WB: 1:2,500 - 1:10,000 dilution Published Applications: see references |
| 供应商 | Active Motif | |
| 纯化方式 | None | |
| 免疫原 | This Histone H3 acetyl Lys18 antibody was raised against a peptide including acetyl-lysine 18 of histone H3. | |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Acetylation of histones is linked to a number of specific processes including transcriptional regulation and genomic organization. | |
| 运输条件 | Blue Ice | |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. | |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3 acetyl Lys18 antibody tested by ChIP. Top Panel: Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µl of Histone H3 acetyl Lys18 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method. Bottom Panel: Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and 50 µl of Ready-to-ChIP HeLa Chromatin (Catalog No. 53015) per ChIP. Subsequent to the ChIP reaction, DNA was purified from the immunoprecipitated chromatin and a region of the human GAPDH promoter was amplified by PCR. Lane 1: ChIP using negative control rabbit IgG. Lane 2: ChIP using 10 µl of Histone H3 acetyl Lys18 antibody. Lane 3: PCR input control. | |
Histone H3 acetyl Lys18 antibody tested by immunofluorescence. Top left: HeLa cells stained with Histone H3 acetyl Lys18 antibody (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images. | |
Histone H3 acetyl Lys18 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys18 antibody for acetyl Lys18 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: acetyl-Lys4 peptide. Lane 2: unmodified Lys4 peptide. Lane 3: acetyl-Lys18 peptide. Lane 4: unmodified Lys18 peptide. Lane 5: acetyl-Lys9 peptide. Lane 6: acetyl-Lys14 peptide. Lane 7: acetyl-Lys18 peptide (this is a different peptide sequence as the one in lane 3 which also contains acetyl-Lys18). Lane 8: acetyl-Lys23 peptide. Lane 9: acetyl-Lys27 peptide. |
