| 货号 | 39573 |
| 同种亚型 | Serum |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:1,000 dilution Published Applications: WB See references for more information. Individual optimization may be required. Depending upon the gel system employed, a change in mobility of the clipped form of histone H3 may not be observed. |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This Histone H3.cs1 antibody was raised against a peptide containing the site of Cathepsin L cleavage at the N-terminus. |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Histone H3 is proteolytically cleaved at its N-terminus by Cathepsin L during the differentiation of embryonic stem cells. It is possible that this processing occurs in order to remove modifications that serve as protein recognition elements (e.g. Histone H3 trimethyl Lys9) present in the extreme N-terminus. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 16 kDa |
| 参考文献 |
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Histone H3.cs1 pAb tested by Western blot. Untreated (lane 1) and Cathepsin L-treated (lane 2) recombinant His-tagged (C-terminal) histone H3.2 were blotted with Histone H3.cs1 pAb (top panel) or an antibody that detects the His-tag (bottom panel). Only the Cathepsin L-cleaved histone H3 is detected with the H3.cs1 antibody. |
