| 货号 | 39385 |
| 同种亚型 | Serum |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Dot Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:2,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This Histone H4 monomethyl Lys31 antibody was raised against a peptide containing monomethyl Lys31 of human histone H4. |
| 背景 | Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 8 kDa |
| 参考文献 |
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Histone H4 monomethyl Lys31 pAb tested by Western blot. HeLa cell nuclear extract (20 µg per lane) was probed with Histone H4 monomethyl Lys31 pAb at a dilution of 1:1,000. | |
Histone H4 monomethyl Lys31 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4 monomethyl Lys31 pAb for monomethyl Lys31 histone H4. Methylated peptides corresponding to the immunogen were spotted onto PVDF and probed with the antibody at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Unmodified peptide corresponding to amino acids 28-36 of human histone H4 Lane 2: Monomethyl Lys31 Lane 3: Dimethyl Lys31; Lane 4: Trimethyl Lys31 Lane 5: Unmodified peptide corresponding to amino acids 41-49 of human histone H4 Lane 6: Monomethyl Lys44 Lane 7: Dimethyl-Lys44 Lane 8: Trimethyl Lys44. No detection of peptides (mono-, di-, or trimethylated) corresponding to lysine 4, 9, 18, 23 or 27 of histone H3 was observed with this antibody (data not shown). |
