| 货号 | 39271 |
| 同种亚型 | Serum |
| 反应种属 | Budding Yeast |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Dot Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:2,000 dilution Published Applications: see references |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This Histone H2A phospho Ser129 antibody was raised against a peptide including phospho-serine 129 of budding yeast Histone H2A. |
| 背景 | Histone H2A is one of the core components of the nucleosome, the basic building block of chromatin. Unlike mammals that have a distinct DNA damage-responsive histone H2Avariant,H2AX, budding yeast histone H2A contains an SQ motif in the C-terminus that is phosphorylated by the Mec1 and Tel1 kinases in response to DNA double strand breaks. As with mammalian H2AX phosphorylation, phosphorylation of yeast histone H2Aatserine 129 is required for the efficient repair of DNA double-strand breaks. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 14 kDa |
| 参考文献 |
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Histone H2A phospho Ser129 pAb tested by Western blot. Whole-cell extract of yeast exposed to 200Gy ionizing radiation blotted with Histone H2A phospho Ser129 pAb at a dilution of 1:1,000. Lane 1: Yeast containing an alanine at position 129. Lane 2: Wild-type yeast. | |
Histone H2A phospho Ser129 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H2A phospho Ser129 pAb for phospho Ser129 histone H2A. Peptides corresponding to the immunogen and the unmodified version of the immunogen were spotted onto PVDF and probed with the antibody at 1:500. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Phospho-Ser129 peptide. Lane 2: Unmodified Ser129 peptide. |
