| 货号 | 39171 |
| 同种亚型 | Serum |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:4,000 dilution Published Applications: see references For optimal results in Western blotting, primary antibody incubations should be performed overnight at 4°C. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This Histone H4 acetyl Lys8 antibody was raised against a peptide including acetyl-lysine 8 of histone H4. |
| 背景 | Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. The chromatin-remodeling complex SWI/SNF is recruited to promoters through the interaction of the bromodomain of the protein Brg-1, belonging to the SWI/SNF complex, and CBP-acetylated histone H4 Lysine 8, leading to a chromatin remodeling. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 8 kDa |
| 参考文献 |
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Histone H4 acetyl Lys8 pAb tested by Western blot. Detection of acetylated Histone H4 by Western blot analysis using 10 µg HeLa acid extracts and Histone H4 acetyl Lys8 pAb at a 1:1,000 dilution. Lane 1: Acid extract of untreated HeLa cells. Lane 2: Acid extract of HeLa cells treated with sodium butyrate. |
