| 货号 | 39121 |
| 别名 | Histone H2B acetyl Lysine 16 |
| 同种亚型 | Serum |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/Western Blot/Dot Blot |
| 使用方法 | Validated Applications: ChIP: 5 - 10 µl per ChIP WB: 1:5,000 - 1:20,000 dilution Published Applications: see references |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This Histone H2B acetyl Lys16 antibody was raised against a peptide including acetyl-lysine 16 of human histone H2B. |
| 背景 | Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H2A and Histone H2B are acetylated in bulk chromatin by p300 and form acetylated Histone H2A/Histone H2B heterodimers. When DNA associates with intact core histone octamers that contain acetylated H2A/H2B dimers, the inhibition of transcriptional initiation significantly decreases, indicating that acetylation of their lysine residues may mediate transcription. The acetylation of Histone H2B at Lys16 is associated with transcriptional activation and is involved in cell survival process. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 15 kDa |
| 参考文献 |
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Histone H2B acetyl Lys16 pAb tested by Western blot. HeLa acid extract (5 µg per lane) was probed with Histone H2B acetyl Lys16 polyclonal antibody (1:20,000 dilution). Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate. | |
Histone H2B acetyl Lys16 pAb tested by dot blot analysis. Specificity Data: Dot blot analysis was used to confirm the specificity of Histone H2B acetyl Lys16 pAb for acetyl-Lys 16 of histone H2B. Decreasing amounts of modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. Lane 1: Peptide acetylated at lysine 5 of H2B. Lane 2: Unmodified lysine 5 peptide. Lane 3: Peptide acetylated at lysine 16 of H2B. Lane 4: unmodified lysine 16 peptide. Lane 5: Peptide acetylated at lysine 120 of H2B. Lane 6: Unmodified lysine 120 peptide. | |
Histone H2B acetyl Lys16 pAb tested by ChIP analysis. ChIP performed on HeLa cell chromatin using 39121. PCR was performed using primers specific for the promoter region of the human GAPDH gene. Lane 1: negative IgG control. Lane 2: ChIP using 10 µl of 39121. Lane 3: Input DNA control. |
