| 货号 | 39117 |
| 同种亚型 | IgG |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 1:500 dilution WB: 1:1,000 - 1:5,000 dilution Published Applications: ICC/IF See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This Histone H2AX phospho Ser139 antibody was raised against a peptide including phospho-serine 139 of histone H2AX. |
| 背景 | Histone H2AX phospho Ser139(H2AX,H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | 10 mM sodium phosphate pH 7.5, 150 mM NaCl, 30% glycerol, 0.035% sodium azide. |
| 计算分子量 | 15 kDa |
| 参考文献 |
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Histone H2AX phospho Ser139 antibody tested by immunofluorescence. HeLa cells stained with Histone H2AX phospho Ser139 antibody (1:500 dilution) using MAX Stain™ Immunofluorescence Tools. The HeLa cells were blocked with MAXblock™ Blocking Medium and stained with Histone H2AX phospho Ser139 antibody. Panel A: Untreated HeLa cells. Panel B: Cells fixed and stained 90 minutes after 3 Gy ionizing radiation treatment. Top images: Cells were stained with DAPI. Middle images: Same cells stained with Histone H2AX phospho Ser139 antibody. Bottom images: Merge of both images above. Images were made using Zeiss Axiovision with equivalent acquisition settings for direct comparison. Note Panel B-middle image, which shows intense nuclear clustering of ionizing radiation-induced phosphorylation of Ser139 of H2AX. In contrast, Panel A-middle image shows no detectable phosphorylated H2AX. | |
Histone H2AX phospho Ser139 antibody tested by Western blot. Western blot: Nuclear extract of U2OS cells (20 µg per lane) probed with Histone H2AX phospho Ser139 polyclonal antibody (1:500 dilution). Lane 1: untreated cells Lane 2: cells treated with camptothecin |
