| 货号 | 39109 |
| 同种亚型 | Serum |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Dot Blot |
| 使用方法 | Validated Applications: WB: 1:2,000 - 1:5,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This Histone H2A acetyl Lys9 antibody was raised against a peptide including acetyl-lysine 9 of human histone H2A. |
| 背景 | Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H2A and Histone H2B are acetylated in bulk chromatin by p300 and form acetylated H2A/H2B heterodimers. When DNA associates with intact core histone octamers that contain acetylated H2A/H2B heterodimers, the inhibition of transcriptional initiation significantly decreases, indicating that acetylation of their lysine residues may mediate transcription. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 14 kDa |
| 参考文献 |
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Histone H2A acetyl Lys9 pAb tested by Western blot. Western blot probed with Histone H2A acetyl Lys9 polyclonal antibody (1:5,000 dilution). Lane 1: 20 ng recombinant histone H2A. Lane 2: 5 µg acid extract of HeLa cells. Lane 3: 5 µg acid extract of HeLa cells treated with sodium butyrate. | |
Specificity data: Dot blot analysis was used to confirm the specificity of Histone H2A acetyl Lys9 pAb for acetyl Lys9 of Histone H2A. Modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a 1:5,000 dilution. The amount of peptide spotted (in picomoles) is indicated next to each row. Lane 1: Peptide acetylated at Lys5 of H2A. Lane 2: Unmodified Lys5 peptide. Lane 3: Peptide acetylated at Lys9 of H2A. Lane 4: Unmodified Lys9 peptide. |
