| 货号 | 91109 |
| 同种亚型 | IgG2a |
| 反应种属 | Other (Wide Range) |
| 来源宿主 | Mouse |
| 应用 | ChIP/Western Blot/Enzyme-linked Immunosorbent Assay |
| 使用方法 | Validated Applications: ChIP: 10 µg per ChIP WB: 0.5 - 2 µg/ml dilution Bead-based ELISA: 7 - 560 ng/ml Application Testing: Parental Monoclonal vs. Recombinant View PDF file |
| 供应商 | Active Motif |
| 纯化方式 | Ni-NTA |
| 免疫原 | This antibody was raised against Active Motifs unique AM-Tag sequence for use with Tag-ChIP-IT® (Cat No. 53022) analysis. |
| 背景 | AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-Tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® Histone H2Av antibody (biotin) was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. The antibody was directly labeled with biotin using the biotin ligase, BirA. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H2A.Z(H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle.In Drosophila, the H2A variant corresponding to H2AZ is H2Av.H2Av is an essential protein in Drosophila and has been implicated in both activation and repression of transcription. H2Av is localized to centromeric heterochromatin in Drosophila and flies lacking H2Av have reduced levels of heterochromatin components at the centromeres. However, H2Av nucleosome distribution throughout the rest of the Drosophila genome correlates with genes that have an open and uniform chromatin architecture at promoter regions. |
| 运输条件 | Blue Ice |
| 存放说明 | Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing. |
| 存储溶液 | Purified IgG in 50mM sodium phosphate pH 8.0, 150mM NaCl, and 100mM imidazole with 30% glycerol and 0.035% sodium azide. AbFlex™ recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on t |
| 计算分子量 | 15 kDa |
| 参考文献 |
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