| 货号 | 91229 |
| 同种亚型 | IgG2a |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 0.5 - 2 µg/mL Application Testing: Parental Monoclonal vs. Recombinant View PDF file |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This antibody was raised against full length human recombinant EHMT2 (G9a) protein. |
| 背景 | AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-Tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex EHMT1 (G9a) antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. EHMT2 (G9a) is a histone methyltransferase that specifically mono- and dimethylates Lys9 of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by initiating recruitment of HP1 proteins to methylated histones. EHMT2 (G9a) also mediates monomethylation of Lys56 of histone H3 (H3K56me1) in G1 phase and regulates DNA replication by promoting the interaction between histone H3 and PCNA. EHMT2 (G9a) also weakly methylates Lys27 of histone H3 (H3K27me). EHMT2 (G9a) is most likely targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. EHMT2 (G9a) may also methylate histone H1. |
| 运输条件 | Blue Ice |
| 存放说明 | Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing. |
| 存储溶液 | Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. |
| 计算分子量 | 160 kDa |
| 参考文献 |
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