| 货号 | 91111 |
| 同种亚型 | IgG2a |
| 反应种属 | Other (Wide Range) |
| 来源宿主 | Mouse |
| 应用 | ChIP/Western Blot/Enzyme-linked Immunosorbent Assay |
| 使用方法 | Validated Applications: ChIP: 10 µg per ChIP WB: 0.5 - 2 µg/ml dilution Bead-based ELISA: 7 - 560 ng/ml Application Testing: Parental Monoclonal vs. Recombinant View PDF file |
| 供应商 | Active Motif |
| 纯化方式 | Ni-NTA |
| 免疫原 | This antibody was raised against Active Motifs unique AM-Tag sequence for use with Tag-ChIP-IT® (Cat No. 53022) analysis. |
| 背景 | AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-Tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® AM-Tag antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. TheAM-Tag antibody is specifically designed for use with Active Motif’s unique AM-Tag sequence in combination with the Tag-ChIP-IT® Kit (Catalog No. 53022). Simply clone your protein of interest into Active Motif’s pAM_1C Empty Vector (Catalog No. 53023), or add the AM-Tag sequence to the C-terminus of your protein expression vector. Following transfection, cell lysates are prepared and screened by Western blot for expression of the AM-Tag fusion protein using the AM-Tag antibody. Optimization may be required to identify the optimal conditions for transfection and protein expression. Once transfection conditions have been optimized, the Tag-ChIP-IT® Kit can be used to isolate chromatin and perform ChIP. The AM-Tag has minimal cross reactivity with mammalian samples to ensure low background signal. The tag is also unstructured, which allows the tag to protrude from the protein of interest for maximum exposure during immunoprecipitation. This increases the enrichment efficiency of low abundance transcription factors for more reliable and consistent ChIP results. Use of the AM-Tag for chromatin immunoprecipitation avoids the need for protein-specific ChIP-validated antibodies. It also enables the analysis of sequence variants, mutations and truncations on gene regulation. |
| 运输条件 | Blue Ice |
| 存放说明 | Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing. |
| 存储溶液 | Purified IgG in 200 mM Hepes pH 7.5, 100 mm NaCl, 50 mM NaOAc, 30% glycerol and 0.035% sodium azide. AbFlex™ recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, |
| 计算分子量 | 15 kDa |
| 参考文献 |
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