| 货号 | 61588 |
| 同种亚型 | IgG |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Dot Blot |
| 使用方法 | Validated Applications: WB: 1:2,000 - 1:10,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This antibody was raised against a synthetic peptide containing acetyl-lysine 37 of human Histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3 acetyl Lys37 antibody (pAb) tested by Western blot. HeLa nuclear extract (20 µg per lane) probed with Histone H3 acetyl Lys37 antibody at a 1:2,500 dilution. Lane 1: no treatment. Lane 2: cells treated with sodium butyrate. | |
Histone H3 acetyl Lys37 (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys37 (pAb) for acetyl Lys37 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a 1:2,500 dilution. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: H3 acetyl-Lys37 peptide. Lane 2: unmodified H3 peptide. Lane 3: H3 acetyl-Lys36 peptide. Lane 4: H3 acetyl-Lys9 peptide. Lane 5: H3 acetyl-Lys14 peptide. Lane 6: H3 acetyl-Lys18 peptide. Lane 7: H3 acetyl-Lys23 peptide. Lane 8: H3 acetyl-Lys27 peptide. Lane 9: H4 acetyl-Lys5 peptide. Lane 10: H4 acetyl-Lys8 peptide. Lane 11: H4 acetyl-Lys12 peptide. Lane 12: H4 acetyl-Lys16 peptide. |
