| 货号 | 61418 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:2,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This antibody was raised against a peptide within the N-terminal region of human Histone H1.0. |
| 背景 | Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Histone H1.0 is a unique variant considered a replacement linker histone which is expressed and incorporated into chromatin in the absence of DNA replication. Histone H1.0 is more highly expressed in cells that are growth inhibited or terminally differentiated. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. |
| 计算分子量 | 30 kDa |
| 参考文献 |
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Histone H1.0 (pAb) tested by Western blot. Nuclear Extract of 3T3-L1 cells (20 µg) following stimulation with a defined hormonal mixture (DMSO, dexamethasone, insulin, and IBMX) were probed with Histone H1.0 (pAb) at a dilution of 1:500. Lane 1: Recombinant human Histone H1.0 (100 ng). Lane 2: Day 0 - preadipocytes. Lane 3: Day 12 - fully differentiated adipocytes. |
