| 货号 | 61414 |
| 同种亚型 | IgG |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Dot Blot |
| 使用方法 | Published Applications: WB See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This antibody was raised against a synthetic peptide containing a 3-phosphohistidine analog at Histidine 18 of human Histone H4. |
| 背景 | Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Phosphorylation of histidines in Histone H4 has been known to occur in liver cells and may be associated with proliferation of hepatocytes during regeneration of the liver following damage. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG containing 30% glycerol and 0.035% sodium azide. This serum was depleted over unmodified peptide before protein A purification. |
| 计算分子量 | 8 kDa |
| 参考文献 |
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Dot blot of Histone H4 phospho Histidine 18 antibody (pAb). Dot blot analysis was used to confirm the specificity of H4H18ph antibody for phosphorylation on Histidine 18 of histone H4. Peptides corresponding to the immunogen and an unmodified sequence were spotted onto PVDF and probed with H4H18ph at a 1:1,000 dilution. The amount of pepetide (picomoles) spotted is indicated next to each row. Lane 1: unmodified peptide. Lane 2: H4H18ph peptide. |
