| 货号 | 61392 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunofluorescence/Dot Blot/Immunocytochemistry |
| 使用方法 | Published Applications: ICC/IF WB DB See references for more information. Individual optimization may be required. For Western blotting, antibody should be incubated overnight at 4°C with 0.5-0.67M NaCl containing PBS with 0.05% Tween 20. For diluting secondary antibodies use a 0.237M NaCl containing PBS with 0.05% Tween 20. In addition, washing steps should be performed with 0.5-0.67M NaCl containing PBS with 0.05% Tween 20. |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This antibody was raised against a synthetic peptide containing biotin-Lys 4 of human Histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation, SUMOylation and biotinylation; these modifications play a major role in regulating gene expression. The biotinylation of Histone H3 on Lys4, Lys9 and Lys18 has been reported to be enriched in repeat regions and may participate in repression of transcriptionally competent genes. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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