| 货号 | 61296 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ChIP: 5 - 10 µg per ChIP ChIP-Seq: 5 - 10 µg each ICC/IF: 0.5 µg/ml dilution ChIP-Seq validation was performed by Active Motifs Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here. Published Applications: ChIP WB See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This MLL / HRX antibody was raised against a recombinant protein corresponding to amino acids 2829-2883 of human MLL. |
| 背景 | MLL (myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila)), also known as Histone-lysine N-methyltransferase HRX, is a histone methyltransferase that plays an essential role in early development and hematopoiesis. Catalytic subunit of the MLL1 /MLL complex, a multiprotein complex that mediates both methylation of ‘Lys-4’ of histone H3 (H3K4me) complex and acetylation of ‘Lys-16’ of histone H4 (H4K16ac). In the MLL1/MLL complex, it specifically mediates H3K4me, a specific tag for epigenetic transcriptional activation. Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity. Has no activity toward histone H3 phosphorylated on Thr-3, less activity toward H3 dimethylated on Arg-8 or Lys-9, while it has higher activity toward H3 acetylated on Lys-9. Required for transcriptional activation of HOXA9. Promotes PPP1R15A-induced apoptosis. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 180 kDa |
| 参考文献 |
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MLL / HRX antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from HeLa cells and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify MLL / HRX binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here. | |
MLL / HRX antibody (pAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with MLL / HRX antibody at a 0.5 µg/ml dilution. |
