| 货号 | 61108 |
| 同种亚型 | IgG |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Dot Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:2,000 dilution For optimal results, primary antibody incubations should be performed at 4°C. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This Histone H1.5 phospho Ser17 antibody was raised against a peptide containing phospho Ser17 of human histone H1.5. |
| 背景 | Thenucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Histone H1.5 is an H1 isotype expressed in somatic cells and is heavily phosphorylated during mitosis. Phosphorylation of H1.5 at serine 17 appears in prometaphase and disappears in telophase. The hyperphosphorylated form of H1.5 is mainly chromatin-bound in metaphase when chromatin is maximally condensed. Phosphorylation of H1.5 at serine 17 can be catalyzed by GSK-3. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 36 kDa |
| 参考文献 |
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Western Blot: HeLa nuclear extract (20 µg per lane) probed with the Histone H1.5 phospho Ser17 antibody (pAb) at a dilution of 1:1,000. | |
Specificity Data: Dot blot analysis was used to confirm the specificity of the Histone H1.5 phospho Ser17 antibody. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: phospho Ser17 peptide. Lane 2: unmodified Ser17 peptide. Lane 3: phospho Thr17 histone H1.4 peptide. |
