| 货号 | 39926 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot/Immunofluorescence/Dot Blot/Immunocytochemistry |
| 使用方法 | Validated Applications: ChIP: 5 - 10 µg per ChIP ChIP-Seq: 5 - 10 µg each ICC/IF: 1 - 2 µg/ml dilution WB: 1 - 2 µg/ml dilution Published Applications: see references |
| 供应商 | Active Motif | |
| 纯化方式 | Protein A Chromatography | |
| 免疫原 | This Histone H4 pan-acetyl antibody was raised against a peptide containing the amino terminal region of Tetrahymena histone H2A acetylated at multiple lysines. (The immunogen has a high degree of homology to mammalian histone H4, so the antibody recognizes acetylated histone H4 in HeLa extracts). | |
| 背景 | Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine N-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H4 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as Hat1 or Gcn5. Acetylation of histones is often associated with transcriptional activation. | |
| 运输条件 | Blue Ice | |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. | |
| 存储溶液 | Purified IgG in PBS (pH 7.5) with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an 计算分子量 | 8 kDa |
| 参考文献 |
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Histone H4ac (pan-acetyl) antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 15 ug of chromatin from a human medulloblastoma cell line and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 12 million sequence tags were mapped to identify Histone H4ac (pan-acetyl) binding sites. The image shows binding across a region of chromosome 12. | |
Histone H4ac (pan-acetyl) antibody (pAb) tested by ChIP. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 3 µg of Histone H4 pan-acetyl antibody or the equivalent amount of rabbit IgG as a negative control. Real-time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method. | |
Histone H4ac (pan-acetyl) antibody (pAb) tested by immunofluorescence. HeLa cells stained at 2 µg/ml with Histone H4 pan-acetyl antibody. Top panel: Histone H4 pan-acetyl antibody. Middle panel: DAPI. Bottom panel: merge. | |
Histone H4ac (pan-acetyl) antibody (pAb) tested by Western blot. HeLa nuclear extract (20 µg per lane) probed with Histone H4 pan-acetyl (1 µg per ml). Lane 1: no treatment. Lane 2: cells treated with sodium butyrate. | |
Histone H4ac (pan-acetyl) antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4 pan-acetyl antibody for acetyl histone H4. An acetylated peptide corresponding to the immunogen (Lane 1) as well an unacetylated histone H4 peptide (Lane 2) were spotted onto PVDF and probed with Histone H4 pan-acetyl antibody at 1 µg /ml. The amount of peptide (picomoles) spotted is indicated next to each row. |
