| 货号 | 39916 |
| 同种亚型 | IgG |
| 反应种属 | Budding Yeast, Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot/Immunofluorescence/Dot Blot/Immunocytochemistry |
| 使用方法 | Validated Applications: ChIP: 3 µg per ChIP ChIP-Seq: 3 µg each ICC/IF: 1 - 2 µg/ml dilution WB: 0.5 - 2 µg/ml dilution Published Applications: ChIP WB See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif | |
| 纯化方式 | Protein A Chromatography | |
| 免疫原 | This Histone H3 trimethyl Lys4 antibody was raised against a peptide including trimethyl-lysine 4 of histone H3. | |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4. | |
| 运输条件 | Blue Ice | |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. | |
| 存储溶液 | Purified IgG in PBS (pH 7.5) with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3K4me3 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from a human breast cancer cell line (3 million cells) and 3 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 24 million sequence tags were mapped to identify H3K4me3 binding sites. The left panel shows the presence of the H3K4me3 in the promoters of 5 genes across a 175,000 bp region on chromosome 11. The image in the right panel is zoomed in to show binding in the promoter of a single gene. Binding at the PPFIA1 gene shows the common H3K4me3 double peak with depletion at the transcriptional start site. | |
Histone H3 trimethyl Lys4 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 3 µg of Histone H3 trimethyl Lys4 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method. | |
Histone H3 trimethyl Lys4 antibody tested by immunofluorescence. HeLa cells stained at 2 µg/ml with Histone H3 trimethyl Lys4 antibody. Top panel: Histone H3 trimethyl Lys4 antibody. Middle panel: DAPI. Bottom panel: merge. | |
Histone H3 trimethyl Lys4 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (20 µg) probed with Histone H3 trimethyl Lys4 antibody (1 µg/ml dilution). | |
Histone H3 trimethyl Lys4 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 trimethyl Lys4 antibody for trimethyl-Lys4 of histone H3. Peptides corresponding to regions around major sites of histone H3 methylation were spotted onto PVDF and probed with Histone H3 trimethyl Lys4 antibody at a dilution of 1 µg/ml . The amount of peptide (in picomoles) spotted is indicated next to each row. Top panel: Lane 1: unmodified Lys4. Lane 2: monomethyl Lys4. Lane 3: dimethyl Lys4. Lane 4: trimethyl Lys4. Lane 5: unmodified Lys9, 14, 18. Lane 6: monomethyl Lys9. Lane 7: dimethyl Lys9. Lane 8: trimethyl Lys9. Lane 9: dimethyl Lys14. Lane 10: monomethyl Lys18. Lane 11: dimethyl Lys18. Lane 12: trimethyl Lys18. Bottom panel: Lane 1: Unmodified Lys23. Lane 2: Monomethyl Lys23. Lane 3: Dimethyl Lys23. Lane 4: Trimethyl Lys23. Lane 5: unmodified Lys27. Lane 6: monomethyl Lys27. Lane 7: dimethyl Lys27. Lane 8: trimethyl Lys27. Lane 9: unmodified Lys36. Lane 10: monomethyl Lys36. Lane 11: dimethyl Lys36. Lane 12: trimethyl Lys36. |
