| 货号 | 39798 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | ChIP/Western Blot |
| 使用方法 | Validated Applications: ChIP: 10 µl per ChIP WB: 1:500 - 1:2,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This AIB1 antibody was raised against a peptide derived from human AIB1/SRC-3. |
| 背景 | AIB1(Amplified in Breast Cancer 1,Steroid Receptor Coactivator-3,Nuclear Receptor Coactivator 3,SRC-3,NCOA3) is a chromatin-associated protein that interacts with nuclear hormone receptors to modify their activity as transcriptional activators. AIB1 has histone acetyltransferase (HAT) activity and recruits p300/CBP-associated factor (PCAF) and CREB binding protein (CBP) as part of a multisubunit co-activator complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. It is Involved in the coactivation of different nuclear receptors, such as GR, ER, RARs, RXRs, TRs, VDR and PPARs. It is also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFκB1 protein. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 160 kDa |
| 参考文献 |
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AIB1 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and MCF-7 chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µl of AIB1 / SRC-3 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the pS2 gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method. | |
AIB1 antibody (pAb) tested by Western blot. Detection of AIB1 by Western blot. Lane 1: HeLa whole-cell extract (30 µg). Lane 2: Whole cell extract (30 µg) of MCF-7 cells. Both probed with AIB1 antibody at a 1:500 dilution. |
