| 货号 | 39710 |
| 同种亚型 | IgG |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Dot Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:2,000 dilution Published Applications: see references |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This Histone H3 dimethyl Arg17 asymmetric antibody was raised against a peptide containing dimethyl-Arg17 (asymmetric) of human histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 17 kDa |
| 参考文献 |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Histone H3 dimethyl Arg17 asymmetric antibody (pAb) tested by Western blot. HeLa nuclear extract (25 µg) probed with Histone H3 dimethyl Arg17 asymmetric antibody at a 1:500 dilution. | |
Histone H3 dimethyl Arg17 asymmetric pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Arg17 asymmetric pAb for dimethyl Arg17 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 dimethyl Arg17 asymmetric pAb at a dilution of 1:30,000. The amount of peptide (picomoles) spotted is indicated next to each row. Top panel Lane 1: unmodified Arg2. Lane 2: monomethyl-Arg2 H3. Lane 3: dimethyl-Arg2 H3 (sym). Lane 4: dimethyl-Arg2 H3 (asym). Lane 5: unmodified Arg8. Lane 6: monomethyl-Arg8 H3. Lane 7: dimethyl-Arg8 H3 (sym). Lane 8: dimethyl-Arg8 H3 (asym). Bottom panel Lane 1: unmodified Arg17. Lane 2: monomethyl-Arg17 H3. Lane 3: dimethyl-Arg17 H3 (asym). Lane 4: dimethyl-Arg17 H3 (sym). Lane 5: unmodified Arg26. Lane 6: monomethyl-Arg26 H3. Lane 7: dimethyl-Arg26 H3 (asym). Lane 8: dimethyl-Arg26 H3 (sym). |
