| 货号 | 39706 |
| 同种亚型 | IgG |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/Western Blot/Dot Blot |
| 使用方法 | Validated Applications: WB: 1:500 dilution Published Applications: ChIP WB See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein A Chromatography |
| 免疫原 | This Histone H4 dimethyl Arg3, asymmetric antibody was raised against a synthetic peptide containing dimethyl-Arg3 (asymmetric) of human histone H4. |
| 背景 | Histone H4 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Asymmetric dimethylation of histone H4 at arginine 3 (Arg3), catalyzed by the PRMT methyltransferases, is associated with transcriptional activation by nuclear hormone receptors. Arg3 methylation has been reported to facilitate the subsequent acetylation of histone H4 by the acetyltransferase p300. Histone H4 dimethyl Arg3 asymmetric methylation (H4R2me2a) is catalyzed by PRMT1 and PRMT6. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 8 kDa |
| 参考文献 |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Histone H4R3me2a (asymmetric) antibody (pAb)tested by Western blot. Nuclear extract of HeLa cells (20 µg) tested by Western blot using Histone H4R3me2a (asymmetric) antibody (pAb) at a dilution of 1:500. | |
Histone H4R3me2a (asymmetric) antibody (pAb)tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4 dimethyl Arg3 asymmetric antibody for dimethyl-arginine 3 of histone H4. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4 dimethyl Arg3 asymmetric antibody at 1:2,500. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: unmodified H4 peptide aa 1-10. Lane 2: H4 monomethyl-Arg3 peptide. Lane 3: dimethyl-Arg3(asymmetric) H4 peptide. Lane 4: dimethyl-Arg3 (symmetric) H4 peptide. | |
Histone H4R3me2a (asymmetric) antibody (pAb) specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H4R3me2a antibody was applied at a dilution of 1:20,000 to Active Motifs MODified™ Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 16 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen. |
