| 货号 | 39694 |
| 同种亚型 | Serum |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/Western Blot/Immunofluorescence/Dot Blot/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 1:100 - 1:500 dilution WB: 1:500 - 1:2,000 dilution Published Applications: ChIP See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This Histone H3 acetyl Lys18 antibody was raised against a peptide including acetyl-lysine 18 of histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Acetylation of histones is linked to a number of specific processes including transcriptional regulation and genomic organization. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3 acetyl Lys18 pAb tested by immunofluorescence. Left: HeLa cells stained with Histone H3 acetyl Lys18 pAb. Right: DAPI stain. | |
Histone H3 acetyl Lys18 pAb tested by Western blot. HeLa nuclear extract (20 µg per lane) probed with Histone H3 acetyl Lys18 pAb (1:2,000 dilution). Lane 1: Untreated cells. Lane 2: Cells treated with sodium butyrate. | |
Histone H3 acetyl Lys18 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys18 pAb for acetyl Lys18 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:500. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: acetyl-Lys4 peptide. Lane 2: acetyl-Lys9 peptide. Lane 3: acetyl-Lys14 peptide. Lane 4: acetyl-Lys18 peptide. Lane 5: acetyl-Lys23 peptide. Lane 6: acetyl-Lys27 peptide. Lane 7: acetyl-Lys36 peptide. Lane 8: acetyl-Lys37 peptide. Lane 9: acetyl-Lys64 peptide. Lane 10: acetyl-Lys79 peptide. |
