| 货号 | 39690 |
| 同种亚型 | IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 1:500 - 1:1,000 dilution WB: 1:500 - 1:2,000 dilution Published Applications: see references |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This Histone H2AX antibody was raised against a peptide derived from the C-terminus of human histone H2AX. |
| 背景 | Histone H2AX(H2AX,H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 15 kDa |
| 参考文献 |
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Histone H2AX antibody tested by immunofluorescence. Detection of Histone H2AX by immunofluorescence. HeLa cells were stained with Histone H2AX antibody at a dilution of 1:1,000. Top panel: Histone H2AX antibody staining. Middle panel: DAPI. Bottom panel: merge. | |
Histone H2AX pAb tested by Western blot. Western blot probed with Histone H2AX pAb at a dilution of 1:2,000. Lane 1: Recombinant human histone H2AX (100 ng). Lane 2: Mouse embryonic fibroblast (MEF) cell wild type nuclear lysate. Lane 3: MEF cell nuclear extract derived from H2AX -/- nulls. |
