| 货号 | 39674 |
| 同种亚型 | Serum |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Dot Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:2,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This Histone H3 monomethyl Arg8 antibody was raised against a peptide containing monomethyl-Arg8 of human histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone H3 Arg8, methylated by PRMT enzymes, is involved in nuclear-receptor-mediated transcriptional activation. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3 monomethyl Arg8 pAb tested by Western blot. HeLa cell nuclear extract (20 µg) probed with Histone H3 monomethyl Arg8 pAb (1:500 dilution). | |
Histone H3 monomethyl Arg8 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 monomethyl Arg8 pAb for monomethyl-arginine 8 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 monomethyl Arg8 pAb at 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row. Top Panel: Lane 1: unmodified Arg2 peptide. Lane 2: monomethyl-Arg2 H3 peptide. Lane 3: dimethyl-Arg2 H3 (symmetric) peptide. Lane 4: dimethyl-Arg2 H3 (asymmetric) peptide. Lane 5: unmodified Arg8 peptide. Lane 6: monomethyl-Arg8 H3 peptide. Lane 7: dimethyl-Arg8 H3 (symmetric) peptide. Lane 8: dimethyl-Arg8 H3 (asymmetric) peptide. Bottom Panel: Lane 1: unmodified Arg17 H3 peptide. Lane 2: monomethyl-Arg17 peptide. Lane 3: dimethyl-Arg17 H3 (symmetric) peptide. Lane 4: dimethyl-Arg17 H3 (asymmetric) peptide. Lane 5: unmodified Arg26 peptide. Lane 6: monomethyl-Arg26 H3 peptide. Lane 7: dimethyl-Arg26 H3 (symmetric) peptide. Lane 8: dimethyl-Arg26 H3 (asymmetric) peptide. |
