| 货号 | 39670 |
| 同种亚型 | Serum |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/Western Blot/Dot Blot |
| 使用方法 | Validated Applications: ChIP: 10 µl per ChIP WB: 1:5,000 - 1:25,000 dilution |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This Histone H2B acetyl Lys12 antibody was raised against a peptide containing acetyl Lys12 of Histone H2B. |
| 背景 | Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Acetylation of histones is linked to a number of specific processes including transcriptional regulation and genomic organization. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 15 kDa |
| 参考文献 |
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Histone H2B acetyl Lys12 pAb tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µl of Histone H2B acetyl Lys12 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the PABPC1 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method. | |
Histone H2B acetyl Lys12 pAb tested by Western blot. A549 nuclear extract (10 µg per lane) probed with Histone H2B acetyl Lys12 pAb (1:5,000 dilution). Lane 1: untreated cells. Lane 2: cells treated with Trichostatin A. | |
Histone H2B acetyl Lys12 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H2B acetyl Lys12 pAb for acetyl-Lys12 of histone H2B. Modified and unmodified peptides were spotted onto PVDF and probed with Histone H2B acetyl Lys12 pAb at a dilution of 1:5,000. The amount of peptide (in picomoles) spotted is indicated next to each row. Lane 1: unmodified lysine 12 peptide. Lane 2: acetyl-lysine 5 peptide. Lane 3: acetyl-lysine 12 peptide. Lane 4: acetyl-lysine 16 peptide. Lane 5: acetyl-lysine 46 peptide. Lane 6: acetyl-lysine 120 peptide. |
