货号 | 39648 |
同种亚型 | IgG |
反应种属 | Budding Yeast |
来源宿主 | Rabbit |
应用 | ChIP/Western Blot |
使用方法 | Validated Applications: ChIP: 2 - 5 µl per ChIP WB: 1:500 - 1:1,000 dilution |
供应商 | Active Motif |
纯化方式 | Affinity Purified |
免疫原 | This Htz1 antibody was raised against full-length Htz1 protein from S. cerevisiae. |
背景 | Htz1 is the S. cerevesiae homolog of histone H2AZ and is involved in transcriptional regulation and chromosome architecture. |
运输条件 | Blue Ice |
存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
计算分子量 | 15 kDa |
参考文献 |
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Htz1 pAb tested by ChIP. Chromatin immunoprecipitation performed on S. cerevesiae chromatin using 2 µl of Htz1 pAb. Real-time quantitative PCR was performed using primers corresponding to the Act1 gene open reading frame and the GAL1 gene TATA box. | |
Htz1 pAb tested by Western blot. Lane 1: S. cerevesiae whole-cell extract probed with Htz1 pAb at a 1:500 dilution. Lane 2: Extract from a wild-type strain probed with Htz1 pAb at a 1:1000 dilution. Lane 3: Extract from a strain deleted for the Htz1 gene probed with Htz1 pAb at a 1:1000 dilution. |