| 货号 | 39594 |
| 同种亚型 | Serum |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ICC/IF: 1:250 - 1:1,000 dilution WB: 1:1,000 - 1:2,500 dilution Published Applications: see references |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This Histone macroH2A1 antibody was raised against recombinant protein corresponding to the N-terminal half of mouse macroH2A1.2, so will react with macroH2A1 and the macroH2A1.1 and macroH2A1.2 isoforms. |
| 背景 | Histone macroH2A(mH2A) is a histone variant that has a region that is similar to histone H2A but has a unique C-terminal domain (the macro domain, also called the non-histone domain (NHD)) in addition to the histone-like region. mH2A associates with condensed chromatin, including the inactive mammalian female X chromosome, senescence-associated heterochromatin foci, imprinted genetic loci, and regions of chromatin that are CpG methylated.In vitro assays with mH2A have shown that nucleosomes containing macroH2A are resistant to ATP-dependent chromatin remodeling and transcription factor binding, and that macroH2A is capable of repressing the initiation of RNA pol II catalyzed transcription. MacroH2A1 consists of two isoforms, macroH2A1.1 and macroH2A1.2, that are produced by alternative splicing; they differ by only 30 amino acids in the C-terminal non-histone domain (NHD). |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 41 kDa |
| 参考文献 |
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Histone macroH2A1 pAb tested by immunofluorescence. Staining of HeLa cells with Histone macroH2A1 pAb (1:1,000 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel). | |
Histone macroH2A1 pAb tested by Western blot. HeLa nuclear extract (20 µg per lane) was probed with Histone macroH2A1 pAb at a dilution of 1:2,000. |
