| 货号 | 39382 |
| 同种亚型 | Serum |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot/Immunofluorescence/Dot Blot/Immunocytochemistry |
| 使用方法 | Validated Applications: ChIP: 5 - 10 µl per ChIP ChIP-Seq: 5 - 10 µl each ICC/IF: 1:500 - 1:2,000 dilution WB: 1:1,000 - 1:2,000 dilution Published Applications: see references |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This Histone H3 acetyl Lys4 antibody was raised against a peptide containing acetyl-lysine 4 of histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone acetylation is often associated with transcriptional activation. Lysine 4 of histone H3 can also be mono-, di- or trimethylated. This methylation is associated with transcriptional activation. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3K4ac antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from a human medulloblastoma cell line and 4 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify Histone H3K4ac binding sites. The image shows binding of GAPDH on chromosome 12. | |
Histone H3K4ac antibody (pAb) tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µl of Histone H3 acetyl Lys4 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method. | |
Histone H3K4ac antibody (pAb) tested by immunofluorescence. Top left: HeLa cells stained with Histone H3 acetyl Lys4 pAb (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images. | |
Histone H3K4ac antibody (pAb) tested by Western blot. Western Blot: A549 whole-cell extract (20 µg per lane) probed with Histone H3 acetyl Lys4 pAb (1:2,000 dilution). Lane 1: Untreated cells. Lane 2: Cells treated with Trichostatin A. | |
Histone H3K4ac antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys4 pAb for acetyl Lys4 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: acetyl lysine 4 peptide. Lane 2: unmodified lysine 4 peptide. Lane 3: acetyl lysine 9,14,18 peptide. Lane 4: unmodified lysine 9,14,18 peptide. Lane 5: acetyl lysine 9 peptide. Lane 6: acetyl lysine 14 peptide. Lane 7: acetyl lysine 18 peptide. Lane 8: acetyl lysine 23 peptide. Lane 9: acetyl lysine 27 peptide. |
