| 货号 | 39166 |
| 同种亚型 | Serum |
| 反应种属 | Budding Yeast, Human, Mouse, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot/Immunofluorescence/Dot Blot/Immunocytochemistry |
| 使用方法 | Validated Applications: ChIP: 2 - 8 µl per ChIP ChIP-Seq: 5 µl each ICC/IF: 1:500 - 1:2,000 dilution WB: 1:5,000 - 1:20,000 dilution Published Applications: ChIP ChIP-Seq WB See references for more information. Individual optimization may be required. The modENCODE and NIH Roadmap Epigenomics Mapping Consortiums have implemented rigorous standardization criteria for all assays and reagents to be used. As part of this initiative, antibody specificity testing and the ability of the antibodies to work in ChIP-Seq were assessed in a large-scale study. This Histone H4 acetyl Lys12 antibody was validated for ChIP-Seq in the study. |
| 供应商 | Active Motif | |
| 纯化方式 | None | |
| 免疫原 | This Histone H4 acetyl Lys12 antibody was raised against a peptide including acetyl-lysine 12 of histone H4. | |
| 背景 | Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. The acetylation of histone H4 Lys12 is carried out by several histone acetyltransferases (HATs).Histone H4 Lys12 acetylation by TIP60 occurs in the nucleus, whereas Hat1-mediated acetylation of histone H4 Lys12 occurs in the cytoplasm, indicating the spatial regulation of histone acetylation. The acetylation of histone H4 Lys12 residue is commonly associated with transcriptional activation. | |
| 运输条件 | Blue Ice | |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. | |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an 计算分子量 | 8 kDa |
| 参考文献 |
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Histone H4 acetyl Lys12 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µl of Histone H4 acetyl Lys12 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method. | |
Histone H4 acetyl Lys12 antibody tested by immunofluorescence. Staining of HeLa cells with Histone H4 acetyl Lys12 antibody (1:500 dilution, top panel) and DAPI (bottom panel). | |
Histone H4 acetyl Lys12 antibody tested by Western blot. HeLa acid extract probed with Histone H4 acetyl Lys12 polyclonal antibody (1:10,000 dilution). Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate. | |
Specificity Data. Top Panel: Dot blot was used to confirm the specificity of Histone H4 acetyl Lys12 antibody for H4 acetyl Lys12. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed at a dilution of 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Histone H4 acetyl-Lys5 peptide. Lane 2: Unmodified Lys5 peptide. Lane 3: Acetyl-Lys8 peptide.Lane 4: Unmodified Lys8 peptide. Lane 5: Acetyl-Lys12 peptide. Lane 6: Unmodified Lys12 peptide. Lane 7: Acetyl-Lys16 peptide. Lane 8: Unmodified Lys16 peptide. Bottom Panel: Western blot (1:2,000 dilution) using whole-cell extracts (35 µg per lane) from wild-type yeast or yeast containing a mutated histone H4 gene. Lane 1: Wild-type H4. Lane 2: H4 N-terminal tail deleted. Lane 3: Histone H4 K5 to R. Lane 4: H4 with K8 to R. Lane 5: H4 with K12 to R. Lane 6: H4 K16 to R. Lane 7: H4 K5, 8 & 12 to R. |
